50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、0.1% Triton® X-100、50% Glycerol、pH 7.1 @ 25°C 10* Reaction Buffer: 200 mM Tris-HCl、100 mM (NH4)2SO4、20mM MgSO4、100 mM KCl、1% Tween® 20、pH 8.8@25°C Magnesium Sulfate (MgSO4) Solution：100mM MgSO4
操作步骤
Incubate the following reaction at 65°C for 30–60 minutes
Component
Final Concentration
10X Isothermal Amplification Buffer II
1X (contains 2 mM MgSO4)
MgSO4 (100 mM)
6 mM (8 mM total)
dNTP Mix (10 mM)
1.4 mM each
FIP/BIP Primers (25X)
1.6 µM
F3/B3 Primers (25X)
0.2 µM
LoopF/B Primers (25X)
0.4 µM
Bst DNA Polymerase (8,000 U/ml)
320 U/ml
DNA or RNA Sample
> 10 copies or more
Nuclease-free Water
to 25 µl
Total Reaction Volume
25 µl
注意事项
1.Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.
2.100 µg/ml BSA or 0.1%Triton X-100 is required for long term storage.
3.Reaction temperatures above 70°C are not recommended.
4.Bst DNA Polymerase, Large Fragment cannot be used for thermal cycle sequencing or PCR
产品数据
生物活性
Experimental design, in a 25μl system, using the same amount (0.1ng) of pCMV-Cre-EGFP as the template, using different amounts of this product or Bst DNA polymerase of Competitor, primer: 1.6µM FIP/BIP, 0.2µM F3/B3, 0.4µM Loop F/B, 1.4mM dNTP each, MgSO4 was added to 1X Bst Reaction Buffer (2mM MgSO4) until the final concentration reached 8mM, and incubated at 65ºC for 1 hour. Inactivation was heated at 80ºC for 20 minutes and then tested by 2.0% agarose gel electrophoresis. As shown in the figure, this product has comparable enzyme activity compared with Competitor N's products. Lane 1 Negative Control-1(negative control with no added enzyme only) Lane 2 Negative Control-2(only negative controls without templates) Lane 3 UA070061- Bst DNA Polymerase, Large fragment 8U Lane 4 competing product N 8U
大包装询价 
营业执照（三证合一） 
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