SPR Service
Using SPR technology to accurately detect the interaction characteristics of multiple target proteins, cytokines, and antibody drug molecules.
Surface plasmon resonance(SPR) is an optical based analysis technology.SPR technology utilizes the resonance between evanescent waves and metal surface plasma waves during total reflection of light, resulting in a significant reduction in reflected light.When the wavelength of the incident light is fixed, the intensity of the reflected light is a function of the incident angle, and the incident angle corresponding to the lowest intensity of the reflected light is the resonance angle.The refractive index of the medium on the metal surface is very sensitive, and when the properties or adhesion of the surface medium change, the resonance angle will be different.
Simply put, SPR is based on the amount of ligands bound to the metal surface or whether they bind to generate different resonance angles.The change in resonance angle is proportional to the number of Ligand Analytes, in order to determine the binding state and Ka(thermodynamic affinity constant) of ligands with Analytes.
Platform Introduction
UA SPR technology platform adopts Biacore T200 and has completed the detection of the affinity and activity of multiple targets and cytokines.The detection molecular categories include but are not limited to FcR receptors, popular target proteins(PD - 1 / PD - L1, CD47, EFGR), cytokines, and various antibodies (monoclonal antibodies, dual antibodies, ADC antibodies).
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Hundred Proteins Accelerate TAT
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Case
Target Protein Affinity
EGFR Fc Chimera, Human (Cat. No. UA010174) captured on Protein A Biosenor, can bind HB-EGF,Human (Cat. No. UA040029) with an affinity constant of 0.39μM as determined in SPR assay.
Receptor Protein
Anti-His antibody Immobilized on CM5 Chip captured CD16 His Tag, Cynomolgus (Cat. No. UA020018), can bind IgG1-Fc (UA050001) with an affinity constant of 0.311μM as determined in SPR assay (Biacore T200).
Antibody and Target Protein
Anti-Human HER2(Trastuzumab) Monoclonal Antibody captured on Protein A Biosenor, can bind Her2 His Tag, Human (Cat. No. UA010176) with an affinity constant of 1.35 nM as determined in SPR assay.
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