Technical Articles

This article focuses on the classification system of human dendritic cells, systematically elaborating on the surface markers, transcriptional characteristics, and immune functions of various blood DC subsets (cDC1, cDC2/cDC3, pDC, CD16+ DC, and AS-DC), while analyzing the subset distribution of lymphoid tissue DCs and tissue DCs.

This article systematically elaborates on the subset-specific surface markers, TLR expression profiles, and cytokine secretion characteristics of human and mouse-derived conventional DCs (cDC1 and cDC2), plasmacytoid DCs, and monocyte-derived DCs, focusing on the classification system of dendritic cells.

This article systematically elaborates on the classification system of dendritic cells, detailing the surface markers, cytokine profiles, and core functional characteristics of conventional DCs (cDC1 and cDC2), plasmacytoid DCs, and monocyte-derived DCs in both human and murine systems.

Cytokines are indispensable core research tools in the fields of immunology, cell culture, and drug development, widely used in various experiments such as cell activation, signaling pathway exploration, and in vivo animal model construction. A recombinant cytokine with stable performance and reliable quality directly determines the reproducibility and reliability of experimental data.

This article systematically elaborates on the in vitro culture requirements of dendritic cells, detailing their developmental origins and subgroup characteristics, while analyzing the technical pathways and operational key points for inducing DC differentiation from various sources such as bone marrow CD34+ hematopoietic stem cells, peripheral blood monocytes, and induced pluripotent stem cells.

This article systematically elaborates on the molecular characteristics and biological functions of Th17 cell cytokines, focusing on their protective roles in host defense (particularly against extracellular bacteria and fungal infections) and their pathogenic effects in autoimmune inflammation, while analyzing the regulatory mechanisms of upstream signals such as IL-6 on Th17 differentiation and function.

This article focuses on the molecular characteristics and biological functions of Th2 cytokines, systematically elaborating their core role in humoral immune responses, and analyzing the pathological significance of Th1/Th2 balance shift in tumor immunity, autoimmune diseases, infectious diseases, and reproductive immunology.

This article focuses on the molecular characteristics and biological functions of Th1 cytokines, systematically elucidating their central role in cellular immune responses, and analyzing the pathological significance of Th1/Th2 balance shift in autoimmune diseases, tumors, and reproductive immunology.

This article focuses on the core requirements of in vitro culture of mouse T cells, systematically elaborating the "three-signal mechanism" necessary for T cell activation and its molecular basis, while analyzing the technical principles and operational key points of solid-phase coating or magnetic bead methods based on anti-CD3/CD28 antibodies.

This article systematically elucidates the dynamic process of human B-cell differentiation into plasma cells, detailing the stage-specific phenotypic changes, immunoglobulin class switching patterns, and nuclear transcription factor expression characteristics during the transition from memory B cells through activated B cells and plasmablasts to mature plasma cells.