IL-2 Protein, Human

生物活性

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  Measured in a cell proliferation assay using CTLL2 cells, the EC50 for this effect is 0.1 ng/ml.

电泳(SDS-PAGE)

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  2μg (R: reducing condition, N: non-reducing condition).

反相高效液相色谱(RP-HPLC)

ELISA

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  Immobilized IL-2 Protein, Human (Cat. No. UA040108) at 0.5μg/mL (100μL/well) can bind IL-2R alpha/CD25 His Tag Protein, Human (Cat. No. UA010367) with EC50 of 46.89-66.25ng/mL.
  

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  Immobilized IL-2 Protein, Human (Cat. No. UA040108) at 0.5μg/mL (100μL/well) can bind IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260) with EC50 of 0.78-1.01ng/mL.
  

SPR

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  Anti-His antibody Immobilized on CM5 Chip captured IL-2R alpha/CD25 His Tag, Mouse (Cat. No. UA010324), can bind IL-2, Human (Cat. No. UA040108) with an affinity constant of 20.57 nM as determined in SPR assay.

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  Anti-His antibody Immobilized on CM5 Chip captured IL-2R alpha/CD25 His Tag, Human (Cat. No. UA010367), can bind IL-2, Human (Cat. No. UA040108) with an affinity constant of 13.94 nM as determined in SPR assay.

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  Anti-His antibody Immobilized on CM5 Chip captured IL-2R beta/CD122 His Tag, Human (Cat. No. UA010372), can bind IL-2, Human (Cat. No. UA040108) with an affinity constant of 0.32 μM as determined in SPR assay.