IFN-α 1b Protein, Human

2μg (R: reducing condition, N: non-reducing condition).

Primary antibody: IFIT1 Recombinant Rabbit mAb (S0B1242) at 1/1000 dilution

Lane 1: untreated HeLa whole cell lysate 20 µg

Lane 2: HeLa treated with 10 ng/ml IFN-α 1b(UA040067) for 16 hours whole cell lysate 20 µg

Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 55 kDa

Observed MW: 58 kDa

Protein G Chip captured IFNAR1 Fc Chimera Protein, Human (Cat. No. UA011167) can bind IFN-α 1b Protein, Human (Cat. No. UA040067) with an affinity constant of 95.80nM as determined in SPR assay.