IFN-α 2a Protein, Human

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The EC₅₀ for this effect is less than 0.2 ng/ml.

Measured by a cytotoxicity assay using TF-1 Cells. The EC₅₀ for this effect is less than 1ng/ml.

2μg (R: reducing condition, N: non-reducing condition).

>95% determined by RP-HPLC.

Primary antibody: IFIT1 Recombinant Rabbit mAb (S0B1242) at 1/1000 dilution

Lane 1: untreated HeLa whole cell lysate 20 µg

Lane 2: HeLa treated with 10 ng/ml IFN-α 2a(UA040038) for 16 hours whole cell lysate 20 µg

Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 55 kDa

Observed MW: 58 kDa

Protein A Chip captured IFN-alpha/beta R1 Fc Chimera Protein, Human (Cat. No. UA011186), can bind IFN-α 2a Protein, Human (Cat. No. UA040038) with an affinity constant of 1.30μM as determined in SPR assay.