EGF Protein, Human

The EC50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2 ng/ml.

2μg（R: reducing conditions, N: non-reducing conditions).

Primary antibody: Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb(S0B0851) at 1/1000 dilution 

Lane 1: untreated A431 whole cell lysate 20 µg 

Lane 2: A431 treated with 100 ng/ml EGF(UA040003) for 5 minutes whole cell lysate 20 µg 

Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution 

Predicted MW: 91 kDa 

Observed MW: 90 kDa

Immobilized EGF, Human (Cat. No. UA040003) at 0.5μg/mL (100μL/well) can bind EGFR Fc Chimera, Human (Cat. No. UA010174) with EC50 of 0.11-0.15μg/ml.

Immobilized EGF, Human (Cat. No. UA040003) at 1.0μg/mL (100μL/well) can bind EGFR His Tag, Rhesus macaque (Cat. No. UA010168) with EC50 of 0.54-1.0μg/ ml.

Immobilized EGF, Human (Cat. No. UA040003) at 1.5μg/mL (100μL/well) can bind EGFR His Tag, Mouse (Cat. No. UA010161) with EC50 of 0.84-1.44μg/ ml.

CM5 Chip captured EGF, Human (Cat. No. UA040003), can bind EGFR His Tag, Human (Cat. No. UA010306) with an affinity constant of 2.01μM as determined in SPR assay.

CM5 Chip captured EGF, Human (Cat. No. UA040003), can bind EGFR His Tag, Rhesus macaque (Cat. No. UA010168) with an affinity constant of 0.53mM as determined in SPR assay.