GM-CSF Protein, Human

EC50 < 0.1ng/ml, measured in a cell proliferation assay using TF-1 cells

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is less than 0.1ng/ml.

GM-CSF, Human，3μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.

Primary antibody: CD209 gamma Recombinant Rabbit mAb (S0B1390) at 1/1000 dilution

Lane 1: untreated THP-1 whole cell lysate 20 µg

Lane 2: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg

Lane 3: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg

Lane 4: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg

Lane 5: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg

Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 46 kDa

Observed MW: 45~55 kDa

Anti-His antibody Immobilized on CM5 Chip captured GM-CSFR α His Tag, Human (Cat. No. UA010189), can bind GM-CSF, Human (UA040002) with an affinity constant of 11.9 nM as determined in SPR assay.