Working with Human IGF-I: Carrier-Protein Reconstitution and Gradient Stimulation in Order
A step-ordered guide: reconstitution with carrier protein, aliquoting, serum starvation and gradient stimulation, with AKT-phosphorylation readout timing that works.
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Keywords: IGF-I usage, carrier protein, reconstitution, serum starvation, AKT phosphorylation, gradient, UA100025
Note: The full article is published in Chinese. Please refer to the Chinese version for complete experimental details, figures and product references. For product inquiries, visit www.ua-bio.com.
This article is reviewed and published by the technical expert team of UA
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