Working with CD40 Fc Chimera: Avoiding Fc-Mediated Interference in B Cell and DC Activation Assays

A practical guide to recombinant CD40/TNFRSF5 Fc chimera: choosing Fc controls, plate-coating versus soluble formats, crosslinking strategy and how to separate CD40-specific signaling from Fc-mediated background.

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Abstract: A practical guide to recombinant CD40/TNFRSF5 Fc chimera: choosing Fc controls, plate-coating versus soluble formats, crosslinking strategy and how to separate CD40-specific signaling from Fc-mediated background.

Keywords: CD40 Fc chimera, Fc interference, crosslinking, B cell activation, dendritic cell, co-culture, UA010194

Note: The full article is published in Chinese. Please refer to the Chinese version for complete experimental details, figures and product references. For product inquiries, visit www.ua-bio.com.

This article is reviewed and published by the technical expert team of UA

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