CHI3L1 Binding Assay Tools: QC Evidence and Platform Parameters of HEK293-Derived Recombinant Antigen
The main challenges of CHI3L1 binding assays are glycosylation-dependent epitopes and endotoxin interference. This article reviews the QC evidence of HEK293-expressed recombinant CHI3L1, platform parameters for ELISA, SPR and cell-based assays, and t
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Abstract: The main challenges of CHI3L1 binding assays are glycosylation-dependent epitopes and endotoxin interference. This article reviews the QC evidence of HEK293-expressed recombinant CHI3L1, platform parameters for ELISA, SPR and cell-based assays, and troubleshooting paths for three common anomalies.
Keywords: CHI3L1 binding assay, YKL-40, recombinant protein, SPR, ELISA, endotoxin, UA019077
Note: The full article is published in Chinese. Please refer to the Chinese version for complete experimental details, figures and product references. For product inquiries, visit www.ua-bio.com.
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