Spike RBD Assays: Coating and Control Design for ACE2 Binding and Antibody Detection
A practical guide to RBD-based ELISA: His-tag oriented coating, plate treatment, ACE2-binding and antibody-detection formats, and control design that avoids hook effects and false negatives.
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Keywords: ACE2 binding, ELISA coating, His tag, plate treatment, control design, hook effect, UA030036
Note: The full article is published in Chinese. Please refer to the Chinese version for complete experimental details, figures and product references. For product inquiries, visit www.ua-bio.com.
This article is reviewed and published by the technical expert team of UA
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