Technical Articles

This article focuses on the molecular characteristics and biological functions of single-chain antibody fragments (scFv), systematically elaborating on their structural basis of assembly from antibody heavy-chain variable regions and light-chain variable regions connected by peptide linkers, analyzing the assembly strategies and affinity advantages of multivalent forms such as scFv monomers, tandem antibodies, diabodies, and trimers, and exploring the preparation process and purification strategies for screening high-affinity scFv using phage display technology.