Technical Articles

A step-by-step SOP for ActRIIA kinase inhibitor screening: enzyme concentration titration, Z' factor acceptance thresholds, positive control LDN-193189 dilution design, luminescence readout linearity and the four most common failure nodes with troubl

Picture the ActRIIB kinase assay as a brake light: the brighter the enzyme activity, the tighter the muscle brake. This article walks through the complete journey of an inhibitor from addition to readout in four acts.

A tool-oriented guide to ActRIIB kinase inhibitor screening: ATP and enzyme concentration settings, control systems and Z' factor interpretation, luminescence interference checks and IC50 fitting considerations.

Glucose uptake is the initial step in cellular energy metabolism and one of the core indicators for assessing cellular metabolic activity and insulin sensitivity. In fields such as metabolic biology, diabetes research, tumor metabolism, and drug discovery, accurately measuring a cell's ability to uptake glucose serves as a critical experimental basis for understanding cellular functional states and the effects of compound interventions.