Technical Articles

This article systematically elaborates on the molecular mechanism of firefly luciferase-catalyzed substrate oxidation and luminescence, as well as its technical advantages as a reporter gene, focusing on the technical principles and experimental systems of luciferase reporter gene assays. It analyzes the construction strategies of reporter plasmids and the design of experimental procedures, and explores the application strategies of this technology in miRNA target gene validation and promoter transcriptional activity regulation research.