Technical Articles

A systematic review of ActRIIA: extracellular ligand-binding domain, constitutively active intracellular kinase, ALK4 recruitment and SMAD2/3 phosphorylation. Covers physiological contexts from FSH rhythm in the pituitary to osteoclast-osteoblast bal

This article systematically elaborates on the central role of kinases in cellular signal transduction and their significant value as drug targets, focusing on the technical principles and methodological evolution of kinase activity assays. It analyzes the advantages of homogeneous detection methods based on ADP quantification over traditional techniques and explores application strategies of kinase activity assays in inhibitor screening and mechanistic studies.