Technical Articles

This article focuses on the molecular characteristics and splicing regulatory mechanisms of the fibronectin EDA and EDB domains, systematically elaborating the structural differences and functional differentiation of the three forms of fibronectin—plasma fibronectin, cellular fibronectin, and fetal fibronectin—analyzing the molecular basis for the overexpression of the EDA and EDB domains in embryonic tissues, placental tissues, and malignant cells, and exploring the clinical value of EDA/EDB as tumor-specific markers and targeted therapeutic targets.

A selection checklist for recombinant human Galectin-3: domain completeness, activity readouts, purity data and how to judge suppliers before comparing prices.

Galectin-3 is a beta-galactoside-binding lectin with CRD and collagen-like domains. This article covers its oligomerization, tumor-immune roles and recombinant-protein applications.

DKK-1 binds LRP5/6 and Kremen to block Wnt signaling. This article explains the brake mechanism, bone and cancer contexts and how recombinant DKK-1 supports Wnt studies.

From target validation to CAR potency release, fluorescent GPC3 reagents support each stage of HCC cell-therapy development. This article maps the chain and the assay needs.

A practical staining guide for directly labeled GPC3: titration, FMO and isotype controls, compensation setup for AF647 and how to read CAR engagement panels cleanly.

GPC3 is a GPI-anchored heparan sulfate proteoglycan turned on in hepatocellular carcinoma. This article explains its membrane logic and why fluorescent labeling enables targeted studies.

A selection guide for mouse CD47 Fc chimera protein: SIRPα-binding validation, Fc format considerations, purity and quality indicators, and acceptance criteria for blockade assays.

CD47 delivers the "do not eat me" signal through SIRPα and is a key innate-immune checkpoint. This article covers the CD47-SIRPα axis and applications of mouse CD47 Fc chimera protein.

This article focuses on the molecular characteristics and tumor biological functions of CUB domain-containing protein 1 (CDCP1), systematically elaborating its structural composition as a type I single-pass transmembrane glycoprotein, the regulatory mechanisms of proteolytic cleavage, and the functional significance of the neo-epitope generated after cleavage. It analyzes the context-dependent dual functional profile of CDCP1 in promoting invasion and metastasis versus suppressing metastasis, and discusses the levels of evidence and key considerations regarding its tumor expression profile, prognostic assessment, and targeted therapeutic development.