Technical Articles

In vitro activation of the classical Wnt/β-catenin pathway has long been constrained by the physicochemical shortcomings of natural ligands: proteins such as Wnt3a suffer from poor water solubility, complex post-translational modifications, and significant batch-to-batch activity variability, making it difficult to ensure the stability of stem cell maintenance and organoid culture protocols.

Gastric organoids, in simple terms, are three-dimensional (3D) miniaturized organs derived from stem cells in laboratory culture dishes. The construction of normal mouse gastric organoids typically begins with the isolation of adult stem cells or embryonic stem cells from mouse gastric tissue. These cells are cultured within a Matrigel matrix containing specific growth factors (such as EGF, Wnt, Noggin, etc.), which precisely simulate the stem cell niche microenvironment of the gastric mucosa in vivo.