Technical Articles
UA100025
Why Human IGF-I Belongs in Serum-Free Media: From Cell Culture to Manufacturing
IGF-I replaced serum in defined media for stem cells, organoids and cultivated-meat platforms. This article traces the formulation logic and scale-up consistency demands.
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- Signaling Pathway
- IGF-I serum-free media
- cell culture
- organoid
- cultivated meat
- batch consistency
- UA100025
Working with Human IGF-I: Carrier-Protein Reconstitution and Gradient Stimulation in Order
A step-ordered guide: reconstitution with carrier protein, aliquoting, serum starvation and gradient stimulation, with AKT-phosphorylation readout timing that works.
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- Signaling Pathway
- IGF-I usage
- carrier protein
- reconstitution
- serum starvation
- AKT phosphorylation
- gradient
- UA100025
Human IGF-I: How 70 Amino Acids Coordinate Growth, Metabolism and Survival
IGF-I is a 70-residue growth factor acting through IGF-1R to drive PI3K-AKT and MAPK signaling. This article explains how one small peptide coordinates three programs.
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- Signaling Pathway
- IGF-I
- IGF-1
- IGF-1R
- insulin-like growth factor
- PI3K AKT
- MAPK
- growth factor
- UA100025
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