Technical Articles

This article focuses on the molecular characteristics and biological functions of FGF-basic (also known as FGF-2/bFGF), systematically elaborating its key role as a core member of the FGF family in promoting the proliferation of various cells, regulating embryonic development, angiogenesis, and tissue repair. It also analyzes the molecular structure and improved thermal stability properties of its 154aa form.

This article systematically elaborates on the molecular characteristics and clinical significance of erythropoietin (EPO), focusing on its synthesis regulation in the kidneys and its core physiological function of maintaining erythrocyte homeostasis by promoting the proliferation and differentiation of erythroid progenitor cells. It also analyzes the standardized application of rhEPO in renal anemia, tumor-related anemia, and bone marrow failure disorders.

This article systematically elucidates the molecular basis of the neuroprotective effects of FGF-10 in the treatment of ischemic stroke, highlighting its multiple mechanisms, including suppressing neuroinflammation, preserving blood-brain barrier integrity, and promoting angiogenesis.

This article systematically elucidates the central role of Activin A in the development and progression of cancer cachexia, focusing on the molecular mechanism by which this cytokine drives skeletal muscle atrophy through inhibiting PGC1α expression in vascular endothelial cells and inducing endothelial dysfunction.

From pluripotent stem cells to functional mast cells, a one-step professional solution

This article systematically elucidates the central role of fibroblast growth factor (FGF) family members in cell culture and the technical challenges posed by their thermal instability. It highlights the principles of thermally stable FGF technology in enhancing stability and bioactivity through protein engineering, and analyzes the unique application value of FGF-10 as a key member in organoid research.

This paper systematically elucidates the molecular mechanisms by which Noggin acts as an antagonist of the bone morphogenetic protein (BMP) signaling pathway and its central role in regulating cell proliferation and differentiation. It introduces a Noggin activity detection method based on the BMP reporter gene system and highlights its critical application value in the long-term culture of organoids.

This article focuses on the in vitro construction and cultivation of mouse lung organoids, systematically detailing the standardized operational procedures from tissue isolation, enzymatic digestion, Matrigel embedding to primary culture. It analyzes key technical points in critical steps such as passaging and expansion, cryopreservation and revival, and explores how to stably obtain lung organoid models with typical cavity structures through precise control of operational steps.

Human fibroblast growth factor 10 (FGF-10) plays an irreplaceable core role in organoid culture systems, where it specifically regulates epithelial-mesenchymal interactions and is widely involved in the construction and homeostasis maintenance of various organoids, including those of the stomach, liver, lung, breast, and prostate.

The biological significance of Noggin as a key antagonist in the BMP signaling pathway.