Technical Articles

This article focuses on the molecular characteristics and biological functions of R-Spondin 1 (RSPO1), systematically elaborating its domain composition as a core member of the R-spondin family. It analyzes the molecular mechanism by which RSPO1 enhances the Wnt/β-catenin signaling pathway through its furin-like domain binding to LGR4/5 receptors and neutralizing RNF43/ZNRF3-mediated ubiquitination degradation of Frizzled receptors.

This article focuses on the molecular characteristics and biological functions of RSPO3 as a core member of the R-spondin family, systematically elucidating its dual mechanisms in regulating angiogenesis through both the canonical Wnt/β-catenin pathway and the non-canonical Gαi1/3-Akt-mTOR pathway.

This article systematically elaborates on the molecular characteristics and biological functions of VEGF165, a major isoform of vascular endothelial growth factor, highlighting its pivotal role as a key regulator of angiogenesis in the development and progression of solid tumors. It also analyzes its clinical application value in broad-spectrum tumor screening, efficacy evaluation, and prognosis monitoring.

This article systematically elaborates on the molecular characteristics and biological functions of FGF-9 as a core member of the fibroblast growth factor family, focusing on its unique secretory properties, high-affinity binding patterns with FGFR receptors, and its critical role in the development and homeostasis maintenance of the central nervous system.

This article focuses on the biological nature of erythropoietin (EPO), systematically elaborating its core role as the sole key hormone regulating erythropoiesis in maintaining the body's oxygen supply, analyzing its medical application value in diseases such as chronic renal anemia, and based on its classification as a prohibited peptide hormone, discussing the health risks associated with its abuse in sports and advancements in detection technologies.

This article focuses on the molecular characteristics and biological functions of epidermal growth factor (EGF), systematically elaborating its core mechanism of activating EGFR to initiate intracellular signaling cascades and promote epidermal cell proliferation and migration. It also analyzes the significant therapeutic effects of EGF in wound healing and its limitations in non-invasive skincare.

This article systematically elaborates on the molecular characteristics and mechanisms of action of keratinocyte growth factor (KGF/FGF-7) as a unique member of the fibroblast growth factor family, analyzes its biological function in specifically regulating epithelial cell proliferation and differentiation through paracrine signaling, and explores its critical role in tissue injury repair and clinical translation potential.

This article systematically elaborates on the central role of the phosphatidylinositol 3-kinase (PI3K) signaling pathway in cellular regulation, analyzes the design principles and technical advantages of ADP detection-based kinase activity assays, and explores the application strategies of biotinylated substrates in PI3K activity analysis.

This study, based on a mouse model of aortic constriction, systematically elucidates the critical role of myeloid cell-specific ErbB3 signaling in the early adaptation of non-ischemic cardiomyopathy. It focuses on analyzing the mechanism by which this signaling pathway mediates cardiac myeloid cell recruitment and promotes non-immune cell proliferation in male mice, while revealing the sex-specific characteristics of acute heart failure induced by ErbB3 signaling deficiency.

Soquelitinib (CPI-818) has advanced to global Phase III trials for PTCL and Phase II for AD, while the first ITK PROTAC (BSJ-05-037 from Peking University) and Pfizer's dual-target topical inhibitor PF-07245303 have also been reported. These developments indicate that ITK is gradually transitioning from an "early-stage target" to the "clinical validation phase"—for teams still evaluating project initiation or building screening platforms, now may be an opportune time to make strategic moves.