Technical Articles

This article systematically elucidates the synergistic mechanism of firefly luciferase and Renilla luciferase in dual-reporter gene design, centered on the technical principles and application framework of the dual-luciferase reporter gene assay system. It analyzes the application strategies of this system in miRNA target gene validation, promoter activity analysis, and signaling pathway research, while discussing key operational points in reporter gene vector construction and experimental procedures.