Technical Articles

This article systematically elaborates on the molecular mechanism of firefly luciferase-catalyzed substrate oxidation and luminescence, as well as its technical advantages as a reporter gene, focusing on the technical principles and application system of the One-luc luciferase assay system. It analyzes the core features of the One-luc system in terms of operational convenience, signal stability, and high-throughput compatibility, and explores its wide applications in gene expression regulation studies, signaling pathway analysis, and drug screening.