Technical Articles

This paper systematically elucidates the molecular mechanisms by which Noggin acts as an antagonist of the bone morphogenetic protein (BMP) signaling pathway and its central role in regulating cell proliferation and differentiation. It introduces a Noggin activity detection method based on the BMP reporter gene system and highlights its critical application value in the long-term culture of organoids.

Noggin is a secreted glycoprotein and a key morphogen antagonist that regulates embryonic development and organ formation.

Gastric organoids, in simple terms, are three-dimensional (3D) miniaturized organs derived from stem cells in laboratory culture dishes. The construction of normal mouse gastric organoids typically begins with the isolation of adult stem cells or embryonic stem cells from mouse gastric tissue. These cells are cultured within a Matrigel matrix containing specific growth factors (such as EGF, Wnt, Noggin, etc.), which precisely simulate the stem cell niche microenvironment of the gastric mucosa in vivo.

Noggin, as an important regulatory factor of the transforming growth factor beta (TGF - β) superfamily, plays a crucial role in embryonic development, tissue homeostasis, and stem cell fate determination by specifically antagonizing the bone morphogenetic protein (BMP) signaling pathway