Technical Articles

2× Fidelis Master Mix is specifically designed for NGS library construction, integrating ultra-high fidelity, rapid hot-start amplification, long-fragment extension capability, and compatibility with complex templates. It effectively reduces amplification error rates, shortens experimental cycles, and enhances system stability, providing efficient and reliable domestic enzymatic tools for genomics research.

In the face of frequent challenges posed by high GC content and complex secondary structures in genome sequencing and molecular cloning, conventional amplification methods often encounter dilemmas of low efficiency and non-specific amplification.

This article addresses the stringent demands for fidelity and amplification length in next-generation sequencing (NGS) and complex molecular cloning, providing an in-depth analysis of the technical advantages of high-fidelity hot-start PCR master mixes.