Technical Articles

This article focuses on the molecular characteristics and biological functions of fibroblast growth factor 23 (FGF-23), systematically elaborating its core regulatory role as a phosphaturic hormone in mineral metabolism disorders in chronic kidney disease, analyzing the interaction mechanisms between FGF-23 and calcium and phosphorus metabolism as well as the clinical significance of the signaling axis formed with Klotho in the progression of CKD, and exploring the application prospects of FGF-23 as a new target for the diagnosis and treatment of CKD.

A tool-oriented guide to MAT1A activity assays: the interference trap of ATP as an intrinsic substrate, Mg2+/K+ system parameters, platform boundaries among absorbance, HPLC and coupled luminescence readouts, and troubleshooting paths for three commo

MAT1A (EC 2.5.1.6) catalyzes methionine and ATP into SAM, the entry enzyme of one-carbon metabolism. This review covers its two-step catalytic mechanism, oligomeric states, division of labor with MAT2A, disease associations and in vitro research esse

MAT1A is the methylation engine of the liver, yet hepatocellular carcinoma cells systematically switch it off and re-express MAT2A. This article dissects the MAT1A/MAT2A switch, its consequence chain and the contest between two intervention routes fr

A tool-oriented guide to PRLR binding experiments: QC evidence for the His&Fc fusion extracellular domain, platform parameters for ELISA, SPR and BLI, affinity interpretation conventions, and troubleshooting paths for three common data anomalies.

Once defined by lactation biology, the prolactin receptor PRLR has re-entered targeted research three decades later on the strength of high expression profiles in luminal breast cancer. This article unpacks the receptor's dual identity and translatio

A systematic review of the prolactin receptor (PRLR), a class I cytokine receptor family member that transmits prolactin signals via the JAK2-STAT5 pathway: receptor structure and isoforms, signaling hierarchy, physiological functions, and the toolki

From mouse to rhesus monkey, NNMT differs in sequence conservation, tissue expression profiles and model fit. This review covers the core dimensions of cross-species comparison and the logic of aligning tool proteins.

Picture the NNMT reaction as an accounting exercise: SAM is the wallet, SAH the IOU, and histone methylation is where the money is spent. This article walks through the bookkeeping and reconciliation of this methyl ledger in four steps.

A tool-oriented guide to non-human primate NNMT research: three layers of alignment—sequence identity verification, kinetic parallel testing and antibody cross-reactivity validation—plus QC interpretation of recombinant rhesus NNMT.