Technical Articles

This article systematically elaborates on the molecular characteristics and biological functions of fibroblast growth factor 21 (FGF-21), highlighting its multifaceted role as a core regulator of energy homeostasis in the liver, skeletal muscle, pancreas, and heart. It analyzes the mechanisms by which FGF-21 exerts metabolic regulation through promoting glucose utilization, fatty acid oxidation, and anti-inflammatory signaling pathways.

This article focuses on the technical principles of kinase activity detection, systematically elaborating on a universal detection strategy centered on ADP generation as the core indicator, analyzing its advantages over traditional methods and its application value in drug screening.

This article systematically elucidates the detection principle of AMP quantitative assay based on chemiluminescence, which converts AMP into ATP through coupled enzymatic reactions and generates light signals using luciferase. It also analyzes its versatile application value in various enzymatic systems, including cyclic nucleotide phosphodiesterase, DNA ligase, and E3 ubiquitin ligase.

This article focuses on the central role of adenosine monophosphate (AMP) as a key metabolite and product of multiple enzymatic reactions, systematically elaborating on the principles, advantages, and application scope of AMP quantitative detection technology based on the chemiluminescence method. It analyzes its unique value in enzyme activity determination, inhibitor screening, and mechanistic studies.

This article systematically elaborates on the molecular pathological basis, genetic characteristics, clinical manifestations, and diagnostic and therapeutic strategies of coagulation factor XI deficiency (hemophilia C), analyzing its key differences from hemophilia A/B.

This paper systematically elucidates the central role of the NADP⁺/NADPH system as coenzyme II in maintaining cellular redox balance and biosynthesis, and analyzes the scientific rationale for its ratio as a key indicator of oxidative stress.

This article systematically elaborates on the expression characteristics of Nicotinamide N-methyltransferase (NNMT) in cancer-associated fibroblasts (CAFs) and its mechanism of depleting the methyl donor S-adenosylmethionine (SAM). It analyzes the molecular pathways by which NNMT drives CAFs differentiation and promotes tumor progression through epigenetic regulation, and explores the potential of targeting NNMT as a tumor stromal therapeutic strategy.

Based on a landmark study employing proteomics and laser capture microdissection, this article systematically elucidates the phenomenon of elevated nicotinamide N-methyltransferase (NNMT) expression in cancer-associated fibroblasts (CAFs) of high-grade serous ovarian cancer and other solid tumors. It analyzes the molecular mechanism by which NNMT drives the CAF phenotype and pro-tumor microenvironment through depletion of the methyl donor SAM and reduction of histone methylation, while exploring the clinical value of NNMT as a prognostic biomarker and therapeutic target.

This article systematically elaborates on the physiological functions of fibroblast growth factor 23 (FGF-23) as a phosphaturic hormone and its pathological significance in chronic kidney disease (CKD). It analyzes key evidence demonstrating that FGF-23 undergoes early alterations in CKD and serves as a sensitive indicator of phosphorus retention. Additionally, it explores the mechanisms by which FGF-23 mediates cardiovascular damage through "off-target" effects and discusses its potential value as a therapeutic target.

The Severe Challenge of Alcoholic Liver Disease and the Research Value of NNMT.