Technical Articles

This article focuses on the molecular characteristics and biological functions of the Leu16 single-chain variable fragment, systematically elaborating on its structural composition, antigen recognition properties, and molecular advantages as a derived fragment of a mouse anti-human CD20 monoclonal antibody, analyzing the targeting value conferred upon Leu16 scFv by CD20 as a stable surface marker of B-cell malignancies, and exploring its application strategies in B-cell-related immunotherapy research, genetic engineering modification, and immunological detection.