Technical Articles

This article focuses on the molecular principles and detection systems of homogeneous chemiluminescence technology, systematically elaborating its chemiluminescence mechanism based on the short-distance diffusion of singlet oxygen energy between two functionalized nanobeads. It analyzes the core technical advantages of this technology in terms of sensitivity, background control, precision, and operational convenience, and explores its application strategies in biomolecular interaction analysis, molecular diagnostics, and high-throughput screening.