Technical Articles

This article focuses on the molecular characteristics and purification principles of His-tagged recombinant proteins, systematically elaborating the structural composition of polyhistidine affinity tags and their application basis in immobilized metal affinity chromatography. It analyzes the technical differences and selection criteria of chelating ligands such as IDA, NTA, and TED, and discusses the effects of His tag position (N-terminal or C-terminal) on protein expression yield, solubility, and purification performance.