Technical Articles

This article systematically elaborates on the molecular basis of using live cell protease activity as a target for cell viability detection, focusing on the technical principles and application systems of cell viability fluorescence assay kits. It analyzes the technical advantages of fluorescence methods over traditional colorimetric methods in terms of sensitivity, operational convenience, and compatibility with multiplex detection. Additionally, it explores the widespread applications of this assay method in cell proliferation analysis, drug toxicity evaluation, and high-throughput screening.