Technical Articles

This article systematically elaborates on the technical principles and application systems of the bioluminescent luciferase assay system, detailing the molecular mechanism of luciferase-catalyzed substrate oxidation and luminescence, as well as its technical advantages as a reporter gene. It analyzes the synergistic application strategies of firefly luciferase and Renilla luciferase in dual-reporter gene systems and explores the widespread use of this assay system in gene expression regulation studies, signaling pathway analysis, and high-throughput drug screening.