Technical Articles

This article systematically elucidates the molecular characteristics of BRD4's second bromodomain (BD2) recognizing histone H4 acetylation modifications and its functional role in gene transcription regulation and tumorigenesis, centered on the technical principles and application systems of the BRD4(BD2)/H4 binding detection kit. It analyzes the detection principle and signal generation mechanism based on homogeneous time-resolved fluorescence resonance energy transfer (HTRF) technology and explores the application strategies of this kit in the screening of BRD4 isoform-selective inhibitors and high-throughput drug discovery.