Technical Articles

This article systematically elucidates the molecular basis of BRD4 bromodomain recognition of histone H4 acetylation modifications and its central role in gene transcription regulation, focusing on the technical principles and application systems of the BRD4(BD1)/H4 binding detection kit. It analyzes the detection principle and signal generation mechanism based on homogeneous time-resolved fluorescence resonance energy transfer (HTRF) technology and explores the application strategies of this kit in evaluating small molecule-mediated protein-peptide interactions and high-throughput screening.