Technical Articles

This article systematically elaborates on the operational processes and core characteristics of the two main technological approaches—mass spectrometry and DNA/RNA sequencing—in peptide sequencing, analyzing the technical essentials of key steps such as enzymatic digestion, ionization detection, and sequence interpretation. It explores the applicability of different sequencing strategies in proteomics research and introduces the application value of recombinant trypsin in peptide sequencing sample preparation.